The objective of this study was to assess the effect of applying N and K2O at different times after harvest on the structure and production characteristics of Marandu and Xaraés cultivars of Brachiaria brizantha. Two greenhouse 4 × 2 factorial experiments using a randomized block design with eight treatments and four replicates were carried out. In experiment 1, the plants in pots were fertilized weekly with 50 mg/dm³ of N and K2O until a week before a uniformity cut, for a total of four applications. After cutting, plants were fertilized with 50 mg/dm³ of N and K2O at 0, 3, 6, and 9 days. Cultivars were evaluated after 44 days of regrowth. In experiment 2, a single 50 mg/dm³ dose of N and K2O was applied a week before the uniformity cut, and 100 mg/dm³ of N and K2O were applied at 0, 4, 8, and 12 days after cutting; cultivars were evaluated after 39 days of regrowth. In experiment 1, the leaf/stem ratio and dry matter yield were influenced by the time at which fertilizers were applied. In the second experiment, the total number of leaves, expanded leaves, dead leaves, and the leaf lamina accumulated length and dry matter production were influenced by the time at which fertilizers were applied. Marandugrass had more leaves and tillers than cv. Xaraés, which, in turn, had greater height, dry matter yield and regrowth vigor. Applying nitrogen and potassium fertilizer immediately after cutting improves the structural characteristics, while the dry matter yield is maximized by applying fertilizers immediately after cutting by using lower doses of N, or at 4.5 days after cutting by using higher doses of N.